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PHC2 抗体-PHC2 Antibody
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MBP-1.
- 保存条件:
For short periods of storage (days) store at 4℃
- 保质期:
1年
- 级别:
科研级别
- 库存:
100
- 供应商:
艾美捷科技
- 宿主:
兔
- 应用范围:
E, WB
- 抗体英文名:
MBP-1 Antibody
- 抗体名:
MBP-1 Antibody
- 规格:
100ul
MBP-1 抗体,MBP-1 Antibody,MBP-1 抗体,MBP-1 Antibody
产品名称:MBP-1 抗体-MBP-1 Antibody
产品货号:PSI-31-235-100ul
产品规格:100ul
背景资料:MBP-1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The product of this gene may play a role in the suppression of prostate cancer cell growth by regulating the MEK5-mediated signaling pathway.
保存建议:MBP-1 抗体-MBP-1 AntibodyFor short periods of storage (days) store at 4℃
应用类型:E, WB
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文献和实验Factor Xa Cleavage of MBP-Fusion protein
INTRODUCTION In many cases the cleavage can be performed using the free intact fusion, or in same cases with the fusion protein bound to a matrix. The amount of factor Xa, temperature and length of incubation must be calibrated
Purification of MBP (maLTose-binding proteins) Fused Proteins
. Resuspend pellet in 100 ml buffer for analysis on SDS-PAGE. Elute "unbound" proteins from amylose resin by washing resin with 10 column volumes of 20 mM Tris pH7.4, 200 mM NaCl, 10mM b ME, 1 mM EDTA. Elute bound MBP fusion protein
Protein Expression and Purification of MBP-T antigen (131-259)
Comments: T antigen (131-259)is an MBP-Hisx6-fusion protein constructed by Laura Mizoue (refer to the wisdom page for details on the construct). i.Pick a single colony from freshly transformed cells (BL21 (DE3)is the expression host)into 2 ml of LB
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