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- ProSci
- 美国
- PSI-22-352-50uL
- 2025年07月06日
- WB,IHC
- 兔
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human H2AFZ (NP_002097.1).
- 保存条件：
Store at -20℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
兔
- 应用范围：
WB,IHC
- 抗体英文名：
Histone H2A.Z Antibody
- 抗体名：
Histone H2A.Z Antibody
- 规格：
50uL
Histone H2A.Z 抗体，Histone H2A.Z Antibody，Histone H2A.Z 抗体,Histone H2A.Z Antibody
产品名称：Histone H2A.Z 抗体-Histone H2A.Z Antibody
产品货号：PSI-22-352-50uL
产品规格：50uL
背景资料：Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
保存建议：Histone H2A.Z 抗体-Histone H2A.Z AntibodyStore at -20℃
应用类型：WB,IHC
点击：Histone H2A.Z 抗体-Histone H2A.Z Antibody查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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