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- ProSci
- 美国
- PSI-14-187-50uL
- 2025年07月12日
- WB,IHC,IF,IP
- 兔
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein of human NFkB p100 / p52
- 保存条件：
Store at -20℃
- 保质期：
1年
- 级别：
科研级别
- 库存：
100
- 供应商：
艾美捷科技
- 宿主：
兔
- 应用范围：
WB,IHC,IF,IP
- 抗体英文名：
NFkB p100 / p52 Antibody
- 抗体名：
NFkB p100 / p52 Antibody
- 规格：
50uL
NFkB p100 / p52 抗体，NFkB p100 / p52 Antibody，NFkB p100 / p52 抗体,NFkB p100 / p52 Antibody
产品名称：NFkB p100 / p52 抗体-NFkB p100 / p52 Antibody
产品货号：PSI-14-187-50uL
产品规格：50uL
背景资料：This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
保存建议：NFkB p100 / p52 抗体-NFkB p100 / p52 AntibodyStore at -20℃
应用类型：WB,IHC,IF,IP
点击：NFkB p100 / p52 抗体-NFkB p100 / p52 Antibody查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多ProSci公司特色试剂盒、特色抗体以及特色试剂等产品评论，请点击查看艾美捷特色产品中心。
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文献和实验the kinases NIK and IKKα. Phosphorylation of p100 results in the processing of the precursor to the p52 subunit and nuclear accumulation of p52-RelB heterodimers. There is significant cross talk since signaling through the LTβR, for instance
◆ Super-shift反应(含激活的目的转录因子的核蛋白＋标记探针＋目的转录因子的特异抗体). 4. 预电泳和电泳： 0.25X TBE在冰上120V 预电泳1小时; 务必换掉预电泳的缓冲液, 用新的0.25X TB低温下150V，电泳约60分钟。 注意横压电泳的时候注意电流的数字变化,记录开始电泳和电泳结束的电流数据的变化! 5. 电转运 在0.5X TBE中390mA电转移40分钟, 注意转运膜的选择, 有一种离子加强型的转运效果比较
A in two separate Eppies and equilibrate 3x with 500µl EBC-buffer (i.e. add EBC buffer, mix gently, spin down and remove supernatant) a. 1x for antibody binding: add 20µl RFP + 400µl EBC-buffer to equilibrated beads and incubate for 1h
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