葡聚糖凝胶 ® G-50*SIGMA*S6022-25g

说明

一般描述

Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

应用

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.[1][2]

Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.[3][2]

特点和优势

Excellent recovery

Minimal sample dilution

Quick and easy to use

法律信息

Sephadex is a registered trademark of Cytiva

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