- $4515
- Leading Biology
- 美国
- PO55342E2
- 2025年07月15日
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- 文献和实验
- 技术资料
- 保存条件：
Stable for 1 year at -5362°C or below from date of shipment.
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
100 ug
性质：
表达系统：E. coli
种属：HIV
氨基酸序：Pro133-Leu363
标签类型：C-His
分子量：The recombinant human HIV-1 Gag-p24 protein comprises 240 amino acids and has a predicted molecular mass of 26.5 kDa as estimated in SDS-PAGE under reducing conditions.
活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
内毒素：Please contact us for more information.
基因名称：
通用名：Gag-p24
蛋白序参考：ACI05538.1
总结：
形式：Lyophilized
纯度：> 95 % as determined by SDS-PAGE.
储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液：Lyophilized from sterile 0.14M PB, 0.5M NaCl, 20% glycerol, 1mM DTT, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
别名：Gag-p24 Protein, HIV
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文献和实验A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay
subtype O, and to HIV-2 and, simultaneously, p24 antigen of HIV-1. To evaluate this assay for daily routine work we compared it with different third-generation assays using sera from uninfected patients and patients with known HIV infection
法的另一大特征是可使用高浓度镁离子令PA tag和NZ-1的结合发生分离，具体来说就是使用10 mM MES・3 M MgCl2（pH 6.0）将已使用并固定化NZ-1的抗体微珠再生利用。在如此温和的条件下再生抗体微珠，可增加抗体微珠的使用次数，大大降低成本。实际上，即使重复以上操作60次，得到的蛋白的量也不会有太大变化1）。图2:.在动物细胞表达系中表达的附加PA tag的蛋白结合量的评价表达有PA tag蛋白的培养上清液（c.cup）和在NZ-1琼脂糖珠上结合标签蛋白的滤过液
(PBMC) by the appearance of p24 gag protein in the culture supernatant. It can be used in a research setting as a measure of viral burden because the number of patient cells required to produce a positive culture is indicative of cellular viral load.
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