Influenza B (B/PHUKET/3073/201

蛋白长度：A DNA sequence encoding the Influenza virus B (B/PHUKET/3073/2013) hemagglutinin (translated amino acids of EPI529345) (Met1-Thr547), termed as HA, was expressed with a polyhistidine tag at the C-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Influenza B

氨基酸序：Met1-Thr547

标签类型：C-His

分子量：The recombinant hemagglutinin of Influenza virus B (B/PHUKET/3073/2013) consists of 543 amino acids and predicts a molecular mass of 59.1 kDa.

活性：Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.5-5 μg/mL for 1%GRBC.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：Harvey rat sarcoma viral oncogene homolog

通用名：HA

蛋白序参考：

总结：The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 20 mM PB, pH 7.0, 500 mM NaCl, 10 % glycerol. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

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