MERS-CoV Spike Protein (S1+S2

蛋白长度：A DNA sequence encoding the MERS-CoV extracellular domain of spike protein (AFS88936.1) (Met1-Trp1297) was fused with a polyhistidine tag at the C-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：MERS-CoV

氨基酸序：Met1-Trp1297

标签类型：C-His

分子量：The recombinant extracellular domain of spike protein MERS-CoV comprises 1291 amino acids and has a predicted molecular mass of 142.52 kDa.

活性：Measured by its binding ability in a functional ELISA.1. Immobilized Sh(2C-EMC)(1-1297)(Cat:PO54704I2) at 10 μg/mL (100 μl/well) can bind biotinylated DPP4 (Cat:10688-HNCH)，The ECof can biotinylated DPP4 (Cat:10688-HNCH) is 0.02-0.04 ug/mL. 2.Immobilized Sh(2C-EMC)(1-1297)(Cat:PO54704I2) at 10 μg/mL (100 μl/well) can bind biotinylated Fc-DPP4 (Cat:PH50951M3)，The ECof can biotinylated Fc-DPP4 (Cat:PH50951M3) is 0.01-0.02 ug/mL.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：

通用名：Spike

蛋白序参考：AFS88936.1

总结：The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

形式：Lyophilized

纯度：> 85 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 20mM Tris, 300mM NaCl, 10% glycerol, pH7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：coronavirus s1 Protein, MERS-CoV; coronavirus s2 Protein, MERS-CoV; coronavirus spike Protein, MERS-CoV; cov spike Protein, MERS-CoV; ncov RBD Protein, MERS-CoV; ncov s1 Protein, MERS-CoV; ncov s2 Protein, MERS-CoV; ncov spike Protein, MERS-CoV; RBD Protein, MERS-CoV; S Protein, MERS-CoV; s1 Protein, MERS-CoV; Spike RBD Protein, MERS-CoV