- $3865
- Leading Biology
- 美国
- PO54524E1
- 2025年07月07日
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- 详细信息
- 技术资料
- 保存条件：
Stable for 1 year at -4544°C or below from date of shipment.
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
100 ug
蛋白长度：A DNA sequence encoding the E.coli FABI (NP_287908.1) (Gly 2-Lys 262) was expressed, with a polyhistide tag at the N-terminus.
性质：
表达系统：E. coli
种属：E. coli
氨基酸序：Gly2-Lys262
标签类型：N-His
分子量：The recombinant E.coli FABI consisting of 276 amino acids and has a calculated molecular mass of 29.5 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
内毒素：Please contact us for more information.
基因名称：
通用名：FABI
蛋白序参考：NP_287908.1
总结：Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to theshort-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.
形式：Lyophilized
纯度：> 98 % as determined by SDS-PAGE.
储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液：Lyophilized from sterile 20mM Tris 0.5M NaCl, 10% glycerol, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
别名：b1288 Protein, E. coli; ECK1283 Protein, E. coli; envM Protein, E. coli; gts Protein, E. coli; JW1281 Protein, E. coli; qmeA Protein, E. coli
性质：
表达系统：E. coli
种属：E. coli
氨基酸序：Gly2-Lys262
标签类型：N-His
分子量：The recombinant E.coli FABI consisting of 276 amino acids and has a calculated molecular mass of 29.5 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
内毒素：Please contact us for more information.
基因名称：
通用名：FABI
蛋白序参考：NP_287908.1
总结：Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to theshort-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.
形式：Lyophilized
纯度：> 98 % as determined by SDS-PAGE.
储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液：Lyophilized from sterile 20mM Tris 0.5M NaCl, 10% glycerol, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
别名：b1288 Protein, E. coli; ECK1283 Protein, E. coli; envM Protein, E. coli; gts Protein, E. coli; JW1281 Protein, E. coli; qmeA Protein, E. coli
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Enoyl-ACP Reductase Protein, E. coli, Recombinant (His Tag)
$3865
