SARS-CoV-2 (P.1) Spike S1+S2 t

蛋白长度：A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F,F817P, A892P, A899P,A942P, K986P, V987P and furin cleavage site mutants) expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant P.1) which emerged in the Brazil.

性质：

表达系统：HEK293 Cells

种属：SARS-CoV-2

氨基酸序：Met1-Gln1208

标签类型：C-His

分子量：The recombinant SARS-CoV-2 Spike S1+S2 consists of 1231 amino acids and predicts a molecular mass of 136.59 kDa.

活性：Immobilized human ACE2 protein (mFc tag)(Cat:PH52212M4) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (P.1) Spike S1+S2 trimer Protein (ECD, His tag)(Cat:PO54506M2), the ECis 5-32 ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：

通用名：Spike

蛋白序参考：

总结：The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

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