Carbonic Anhydrase IX Protein,

蛋白长度：A DNA sequence encoding the canine CA9 (NP_001138646.1) (Met1-Leu410) was expressed with a C-terminal polyhistidine tag.

性质：

表达系统：HEK293 Cells

种属：Canine

氨基酸序：Met1-Leu410

标签类型：C-His

分子量：The recombinant canine CA9 comprises 384 amino acids and has a predicted molecular mass of 42.1 kDa. The apparent molecular mass of the protein is approximately 50 kDa in SDS-PAGE under reducing conditions due to glycosylation.

活性：Measured by its esterase activity.The specific activity is >100 pmoles/min/μg.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：carbonic anhydrase IX

通用名：CA9

蛋白序参考：NP_001138646.1

总结：Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3–) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.

形式：Lyophilized

纯度：> 85 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

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