PD-1 Protein, Cynomolgus, Reco

蛋白长度：A DNA sequence encoding the cynomolgus PDCD1 (NP_001271065.1) (Met1-Gln167) was expressed with a c-terminal Fc region of human IgG1 tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

性质：

表达系统：HEK293 Cells

种属：Cynomolgus

氨基酸序：Met1-Gln167

标签类型：C-hFc & AVI

分子量：The recombinant cynomolgus PDCD1 consists of 396 amino acids and predicts a molecular mass of 44.5 kDa.

活性：Immobilized Cynomolgus CD274 His (Cat:PK53991M2) at 2 μg/mL (100 μL/well) can bind Cynomolgus PD-1 (hFc & AVI Tag), Biotinylated (Cat:PK54058M4), the ECis 30-180 ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：programmed cell death 1

通用名：PDCD1

蛋白序参考：NP_001271065.1

总结：Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

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