Angiopoietin-2 Protein, Cynomo

蛋白长度：A DNA sequence encoding the cynomolgus / rhesus ANGPT2 (XP_005562586.1/XP_001097949.1) (Lys274-Phe495) was expressed with a polyhistidine tag at the N-terminus. Cynomolgus and Rhesus ANGPT2 sequences are identical.

性质：

表达系统：HEK293 Cells

种属：Cynomolgus, Rhesus

氨基酸序：Lys274-Phe495

标签类型：N-His

分子量：The recombinant cynomolgus / rhesus ANGPT2 consists 241 amino acids and predicts a molecular mass of 27.7 kDa.

活性：Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus Angiopoietin-2 His (Cat: PK54050M1) at 2 μg/ml (100 μl/well) can bind Cynomolgus TIE2-hFc (Cat: PK53986M4), the ECof Cynomolgus TIE2-hFc is 15-90 ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：angiopoietin 2

通用名：ANGPT2

蛋白序参考：XP_005562586.1&XP_001097949.1

总结：Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

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