CD25/IL2RA Protein, Cynomolgus

蛋白长度：A DNA sequence encoding the cynomolgus IL2RA (H6WS54) (Met1-Arg213) was expressed with six amino acids (LEVLFQ) at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Cynomolgus

氨基酸序：Met1-Arg213

标签类型：No tag

分子量：The recombinant cynomolgus IL2RA comprises 199 amino acids and has a calculated molecular mass of 22.4 KDa.The apparent molecular mass of the protein is approximately 38-42 KDa respectively in SDS-PAGE.

活性：Immobilized CD25/IL2RA Protein, Cynomolgus, Recombinant(Cat:PK53990M0) at 2 μg/ml (100 μl/well) can bind biotinylated IL2 Protein, Human, Recombinant(ECD, hFc Tag)(Cat:11848-HNAE-B), The ECis 15-90 ng/mL.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：interleukin 2 receptor, alpha

通用名：IL2RA

蛋白序参考：

总结：CD25 (alpha-chain of the IL-2 receptor, or IL2RA), is a type I transmembrane glycoprotein with a signal peptide, an extracellular region, a transmembrane region, and a cytoplasmic domain. IL2RA is expressed on activated T cells and regulatory T cells and is capable of binding IL2 with low affinity by itself. However, a ligand-induced high-affinity heterotrimeric receptor complex is produced when IL2RA is associated non-covalently with the IL2 receptor beta and gamma chain, and subsequently initiates the intracellular signal pathways such as MAPK or JAK/STAT. On dendritic cells (DC), CD25 has been previously regarded as an activation marker, while both murine and human DC can express CD25, they do not express the beta-chain of the IL-2 receptor, which is indispensable for the execution of IL-2 signaling. The IL2RA (CD25) gene is a substantial component of the high-affinity receptor molecule highly expressed by activated T lymphocytes. Recently, a piece of strong evidence was obtained for the involvement of IL-2RA in conferring susceptibility to type 1 diabetes (T1D). Cancer growth and development are associated with the stimulation of the innate immune system, including enhanced interleukin 2 receptor (IL-2R) expression in immune cells and its shedding into the circulation in a soluble form of SIL-2Ralpha. In most hematological malignancies, including different types of leukemias and lymphomas, SIL-2Ralpha is released directly from the surface of neoplastic cells thus reflecting the tumor bulk, turnover, and activity. Several studies have proved that not only lymphoid cancer cells but also some non-lymphoid cancer cells, express IL-2R on their surface. They include malignant melanoma and carcinomas of the kidney, head and neck, esophagus, and lung. Thus, sIL-2Ralpha is elevated in most proliferative disturbances of the hematopoietic system and many solid tumors.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

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