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- 文献和实验
- 技术资料
- 保存条件：
Stable for 1 year at -4006°C or below from date of shipment.
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
1 mg
性质：
表达系统：HEK293 Cells
种属：Cynomolgus
氨基酸序：Met1-Lys745
标签类型：C-hFc
分子量：The recombinant cynomolgus TEK is a disulfide-linked homodimer. The reduced monomer comprises 964 amino acids and has a calculated molecular mass of 107.6 KDa.
活性：Measured by its binding ability in a functional ELISA.Immobilized mouse S1h-3C-mANGPT2 (Cat:PM53027M1) at 10 μg/ml (100 μl/well) can bind Cynomolgus TEK-Fc, The ECof Cynomolgus TEK-Fc is 0.7-1.64 μg/ml.
内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称：TEK tyrosine kinase, endothelial
通用名：TEK
蛋白序参考：
总结：TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature.
形式：Lyophilized
纯度：> 95 % as determined by SDS-PAGE.
储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
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文献和实验One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
For recombinant expression of extracellular matrix (ECM) proteins or their individual domains, the use of transformed mammalian cells offers two major advantages. First, eukaryotic expression can be expected under optimum conditions
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