4-1BB/CD137 Protein, Cynomolgu

蛋白长度：A DNA sequence encoding the cynomolgus / rhesus TNFRSF9 (XP_005544945.1/NP_001253057.1) (Met1-Gln186) was expressed with the Fc region of human IgG1 at the C-terminus. Cynomolgus and Rhesus TNFRSF9 sequences are identical.

性质：

表达系统：HEK293 Cells

种属：Cynomolgus, Rhesus

氨基酸序：Met1-Gln186

标签类型：C-hFc

分子量：The recombinant cynomolgus / rhesus TNFRSF9 consists of 401 amino acids and predicts a molecular mass of 44 kDa.

活性：1.Measured by its binding ability in a functional ELISA. 2.Immobilized cynomolgus / rhesus TNFRSF9-Fc(Cat:PK53980M4) at 10μg/mL (100μL/well) can bind biotinylated human TNFSF9 (Cat:PH52312M3), the ECof biotinylated human TNFSF9 is 20-100ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：tumor necrosis factor receptor superfamily, member 9

通用名：TNFRSF9

蛋白序参考：XP_005544945.1&NP_001253057.1

总结：CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4and CDT cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials. Soluble forms of CD137 (sCD137) are generated by differential splicing. sCD137 can bind to CD137 ligand to antagonize the costimulatory activities of the membrane-bound CD137 and reduce T cell proliferation and IL-2 secretion.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

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