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- 2025年07月15日
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- 文献和实验
- 技术资料
- 保存条件：
Stable for 1 year at -3999°C or below from date of shipment.
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
100 ug
性质：
表达系统：Baculovirus-Insect Cells
种属：Rhesus
氨基酸序：Phe78-Val216
标签类型：N-His
分子量：The recombinant rhesus NKG2D consists of 155 amino acids and has a calculated molecular mass of 18.3 kDa.
活性：1. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch (Cat:PH50911M4), The ECof human ULBP1-Fch (Cat:PH50911M4) is 0.12-0.28 μg/ml. 2. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch (Cat:10759-H03H), The ECof human MICB-Fch (Cat:10759-H03H) is 33.8-79 ng/ml.
内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称：killer cell lectin-like receptor subfamily K, member 1
通用名：KLRK1
蛋白序参考：NP_001028061.1
总结：KLRK1 (Killer Cell Lectin Like Receptor K1) is a Protein Coding gene. NKG2D, also known as CD314, is an immune receptor that consists of two disulfide-linked type II transmembrane proteins with short intracellular proteins incapable to transduce signals. To transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.
形式：Lyophilized
纯度：> 95 % as determined by SDS-PAGE.
储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液：Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
别名：KLRK1 Protein, Rhesus; NKG2-D Protein, Rhesus; NKG2D Protein, Rhesus
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文献和实验One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
For recombinant expression of extracellular matrix (ECM) proteins or their individual domains, the use of transformed mammalian cells offers two major advantages. First, eukaryotic expression can be expected under optimum conditions
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