Stable for 1 year at -3824°C or below from date of shipment.
保质期:
1-2年
供应商:
安诺伦(北京)生物科技有限公司
规格:
1 mg
蛋白长度:A DNA sequence encoding the rat CNTF (P20294) (Met 1-Met 200) was expressed and purified.
性质:
表达系统:E. coli
种属:Rat
氨基酸序:Met1-Met200
标签类型:No tag
分子量:The recombinant rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa. It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.
活性:1. Immobilized rat CNTF (Cat: PR53804E0) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (aa 1-346) (Cat: PR53753I2) with a linear range of 1.56-50.0 ng/ml.2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EDfor this effect is typically 10-100 ng/ml.
内毒素:Please contact us for more information.
基因名称:ciliary neurotrophic factor
通用名:CNTF
蛋白序参考:
总结:Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. It is a polypeptide hormone that has functions in promoting neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. Its actions appear to be restricted to the nervous system. Ciliary neurotrophic factor (CNTF) has biological effects through the activation of a multi-subunit receptor complex, consisting of an extracellular CNTF binding subunit (CNTFα) and two transmembrane signal transduction proteins: glycoprotein gp130 and LIF receptor. CNTF is considered as a potent survival factor of neurons and oligodendrocyteands may be relevant in reducing tissue destruction during inflammatory attacks. CNTF also is a survival factor for neurons of the peripheral sensory sympathetic and ciliary ganglia. It has been reported that CNTF could be an agent that has therapeutic potential and possibly induces differentiation of large multipolar ganglionic phenotype in a subset of progenitors.
形式:Lyophilized
纯度:> 92 % as determined by SDS-PAGE.
储存条件:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Lyophilized from sterile 50mM Tris, 0.1M NaCl, pH 7.5Please contact us for any concerns or special requirements.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.Please refer to the specific buffer information in the hard copy of CoA.