IL-1 alpha/IL1A Protein, Mouse

蛋白长度：A DNA sequence encoding the mature form of mouse IL1A (NP_034684.2) (Ser 115-Ser 270) was expressed, with an initial Met at the C-terminus.

性质：

表达系统：E. coli

种属：Mouse

氨基酸序：Ser115-Ser270

标签类型：No tag

分子量：The recombinant mouse IL1A consists of 157 amino acids and migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions as predicted.

活性：1. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The ECof mouse IL1R1 is 0.13 μg/mL. 2. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells.The EDfor this effect is typically 15-60 ng/mL.

内毒素：Please contact us for more information.

基因名称：interleukin 1, alpha

通用名：IL1A

蛋白序参考：NP_034684.2

总结：IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6, and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13, and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.

形式：Lyophilized

纯度：> 92 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：Il-1a Protein, Mouse