TrkC Protein, Mouse, Recombina

蛋白长度：A DNA sequence encoding the extracellular domain of mouse TrkC (NP_032772.3) (Met 1-Thr 429) was expressed, with a C-terminal polyhistidine tag.

性质：

表达系统：HEK293 Cells

种属：Mouse

氨基酸序：Met1-Thr429

标签类型：C-His

分子量：The secreted recombinant mouse TrkC comprises 409 amino acids and has a calculated molecular mass of 46.1 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.

活性：Immobilized mouse NTRK3-His at 10 μg/ml (100 μl/well) can bind biotinylated human NT3 (Cat:10286-HNA), The ECof biotinylated human NT3 (Cat:10286-HNA) is 0.04-0.08 μg/ml.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：neurotrophic tyrosine kinase, receptor, type 3

通用名：NTRK3

蛋白序参考：NP_032772.3

总结：NT-3 growth factor receptor is also known as neurotrophic tyrosine kinase receptor type 3 or TrkC tyrosine kinase or Trk-C receptor, is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. TrkC/NTRK3 is widely expressed in the developing and adult nervous system. In later embryonic development, TrkC/NTRK3 is expressed in various structures of the CNS including the caudate-putamen, septal nuclei, cerebellum, and brainstem. Other neurotrophins include, neurotrophin-3 and neurotrophin-4. In the PNS, The trkC hybridization appears to correlate, both temporally and spatially, with the outgrowth of axons toward their peripheral targets. TrkC/NTRK3 is widely expressed in the three identified branches of the mammalian nervous system and appears to correlate with the expression of NT-3, its cognate ligand. The apparent colocalization of trkC transcripts with NT-3 raises the possibility this neurotrophin exerts its trophic effects by a paracrine and/or autocrine mechanism. Signaling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in the TrkC encoding gene have been associated with medulloblastomas, secretory breast carcinomas, and other cancers.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：AW125844 Protein, Mouse; Ntrk3_tv3 Protein, Mouse; TrkC Protein, Mouse