SFRP1 Protein, Mouse, Recombin

蛋白长度：A DNA sequence encoding the mouse SFRP1 (AAC53145.1) (Met1-Lys314) was expressed with a polyhistidine tag at the C-terminus.

性质：

表达系统：CHO Stable Cells

种属：Mouse

氨基酸序：Met1-Lys314

标签类型：C-His

分子量：The recombinant mouse SFRP1 consists of 283 amino acids and predicts a molecular mass of 32.6 kDa.

活性：Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The EDfor this effect is typically 0.4-2 μg/mL in the presence of 300 ng/mL of Recombinant Mouse Wnt 3a.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：secreted frizzled-related protein 1

通用名：SFRP1

蛋白序参考：AAC53145.1

总结：Secreted frizzled-related protein 1, also known as sFRP1, is a 35 kDa prototypical member of the SFRP family. SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 3 amino acids in length and contains a cysteine-rich domain (CRD) that shares 3-5% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. The deregulation of this critical developmental pathway occurs in several human tumor entities. Mouse sFRP1 is highly expressed in kidney and embryonic heart, as well as in the eye, where it is principally localized to the ciliary body and the lens epithelium.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：2210415K03Rik Protein, Mouse; AW011917 Protein, Mouse; AW107218 Protein, Mouse; AW742929 Protein, Mouse; sFRP-1 Protein, Mouse