FGF18 Protein, Mouse, Recombin

蛋白长度：A DNA sequence encoding the mouse FGF18 (NP_032031.1) (Met 1-Gly 207) was fused with a polyhistidine tag at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Mouse

氨基酸序：Met1-Gly207

标签类型：C-His

分子量：The secreted recombinant mouse FGF18 comprises 191 amino acids and has a predicted molecular mass of 22.5 kDa. As a result of glycosylation, it migrates as an approximately 30-35 kDa band in SDS-PAGE under reducing conditions.

活性：1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse FGF18 (Cat:PM53096M2) at 10 μg/mL (100 μl/well) can bind Rat FGFR4 (Cat:PR53780M4), The ECof Rat FGFR4 (Cat:PR53780M4) is 0.44 μg/mL.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：fibroblast growth factor 18

通用名：FGF18

蛋白序参考：NP_032031.1

总结：Fibroblast growth factor 18 (FGF18) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that FGF18 is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Experiment datas identified FGF18 as a selective ligand for FGFR3 in limb bud mesenchymal cells, which suppressed proliferation and promoted their differentiation and production of cartilage matrix. FGF18 appears to regulate cell proliferation and differentiation positively in osteogenesis and negatively in chondrogenesis.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：D130055P09Rik Protein, Mouse; FGF-18 Protein, Mouse