CD6 Protein, Mouse, Recombinan

蛋白长度：A DNA sequence encoding the extracellular domain of mouse CD6 (Q91WN5) (Met 1-Gly396) was expressed with a C-terminal polyhistidine tag.

性质：

表达系统：HEK293 Cells

种属：Mouse

氨基酸序：Met1-Gly396

标签类型：C-His

分子量：The secreted recombinant mouse CD6 comprises 391 amino acids and has a calculated molecular mass of 42.6 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 60-70 kDa in SDS-PAGE under reducing conditions

活性：Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×10E4 cells/well are added to mCD6-His coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/ml PHA, approximately > 45% cells will adhere specifically after 60 minutes at 37℃.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：CD6 molecule

通用名：CD6

蛋白序参考：

总结：T-cell differentiation antigen CD6, also known as TP12 and CD6, is a single-pass type I membrane protein which contains threeSRCR domains. CD6 / TP12 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP12 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP12 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP12 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

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