NOTCH1 Protein, Mouse, Recombi

蛋白长度：A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed，with a C-terminal polyhistidine tag.

性质：

表达系统：Baculovirus-Insect Cells

种属：Mouse

氨基酸序：Met1-Gln526

标签类型：C-His

分子量：The secreted recombinant mouse NOTCH1 (aa 1-526) consists of 518 amino acids and has a calculated molecular mass of 55 kDa. It migrates as an approximately 80 KDa band in SDS-PAGE under reducing conditions.

活性：1. Measured by its binding ability in a functional ELISA. 2. Immobilized human DLL4 (cat: PH50250M4) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The ECof biotinylated mouse NOTCH1-his is 40 ng/mL. 3. Immobilized mouse DLL4-his (Cat:PM53444M2) at 10 μg/mL (100 μl/well) can bind biotinylated mouse NOTCH1-his, The ECof biotinylated mouse NOTCH1-his is 30 ng/mL.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：notch 1

通用名：NOTCH1

蛋白序参考：NP_032740.3

总结：NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair the regeneration of skeletal muscles.NOTCH1 is a prevalent signaling pathway in T cell acute lymphoblastic leukemia (T-ALL). The NOTCH signaling pathway is a conserved signaling cascade that regulates many aspects of development and homeostasis in multiple organ systems. The proto-oncogene NOTCH1 is frequently mutated in around 10% of patients with chronic lymphocytic leukemia (CLL). NOTCH1 mutations in oral squamous cell carcinoma (OSCC) frequently occur near the ligand-binding region. These mutations change the domain structure of this protein and affect the ligand-binding activity. When NOTCH1 is activated by ligand binding, the NOTCH1 intracellular domain (NICD) is cleaved from the cell membrane.

形式：Lyophilized

纯度：> 82 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：9930111A19Rik Protein, Mouse; lin-12 Protein, Mouse; Mis6 Protein, Mouse; N1 Protein, Mouse; Tan1 Protein, Mouse