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- 详细信息
- 技术资料
- 保存条件:
Stable for 1 year at -2679°C or below from date of shipment.
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 规格:
50 ug
蛋白长度:A DNA sequence encoding the mouse PRSS2 (NP_033456.1) precursor (Met 1-Asn 246) was expressed, with a C-terminal polyhistidine tag.
性质:
表达系统:HEK293 Cells
种属:Mouse
氨基酸序:Met1-Asn246
标签类型:C-His
分子量:The recombinant pro form of mouse PRSS2 comprises 242 amino acids and has a predicted molecular mass of 26.2 kDa. The apparent molecular mass of rm PRSS2 is approximately 32 kDa in SDS-PAGE under reducing conditions.
活性:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog #27096). The specific activity is >1500 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称:protease, serine 2
通用名:PRSS2
蛋白序参考:NP_033456.1
总结:Trypsin-2, also known as Trypsin II, Anionic trypsinogen, Serine protease 2, PRSS2 and TRY2, is a secreted protein that belongs to the trypsin serine protease family including Trypsin, PRSS1, PRSS2 and PRSS3. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a proteolytically active mature chain (residues 24-247). PRSS2 contains one peptidase S1 domain. It is secreted into the duodenum, hydrolysing peptides into their smaller building blocks, which is necessary for the uptake of protein in the food. It is secreted by the pancreas in the form of inactive zymogen, trypsinogen and cleaved to its active form in the small intestine when the pancreas is stimulated by cholecystokinin through the common activation mechanism.
形式:Lyophilized
纯度:> 92 % as determined by SDS-PAGE.
储存条件:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Lyophilized from sterile PBS, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
别名:Prss2 Protein, Mouse; Ta Protein, Mouse; Tesp4 Protein, Mouse; Try2 Protein, Mouse; TRY8 Protein, Mouse; TRYP Protein, Mouse
性质:
表达系统:HEK293 Cells
种属:Mouse
氨基酸序:Met1-Asn246
标签类型:C-His
分子量:The recombinant pro form of mouse PRSS2 comprises 242 amino acids and has a predicted molecular mass of 26.2 kDa. The apparent molecular mass of rm PRSS2 is approximately 32 kDa in SDS-PAGE under reducing conditions.
活性:Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog #27096). The specific activity is >1500 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称:protease, serine 2
通用名:PRSS2
蛋白序参考:NP_033456.1
总结:Trypsin-2, also known as Trypsin II, Anionic trypsinogen, Serine protease 2, PRSS2 and TRY2, is a secreted protein that belongs to the trypsin serine protease family including Trypsin, PRSS1, PRSS2 and PRSS3. It consists of a signal peptide (residues 1-15), a pro region (residues 16-23), and a proteolytically active mature chain (residues 24-247). PRSS2 contains one peptidase S1 domain. It is secreted into the duodenum, hydrolysing peptides into their smaller building blocks, which is necessary for the uptake of protein in the food. It is secreted by the pancreas in the form of inactive zymogen, trypsinogen and cleaved to its active form in the small intestine when the pancreas is stimulated by cholecystokinin through the common activation mechanism.
形式:Lyophilized
纯度:> 92 % as determined by SDS-PAGE.
储存条件:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Lyophilized from sterile PBS, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
别名:Prss2 Protein, Mouse; Ta Protein, Mouse; Tesp4 Protein, Mouse; Try2 Protein, Mouse; TRY8 Protein, Mouse; TRYP Protein, Mouse
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PRSS2 Protein, Mouse, Recombinant (His Tag)
$3865






