GLO1 Protein, Mouse, Recombina

蛋白长度：A DNA sequence encoding the mouse GLO1 (NP_079650.3) (Ala 2-Ile 184) was expressed, with a polyhistide tag at the N-terminus.

性质：

表达系统：E. coli

种属：Mouse

氨基酸序：Ala2-Ile184

标签类型：N-His

分子量：The recombinant mouse GLO1 consisting of 190 amino acids and has a calculated molecular mass of 21.6 kDa. The apparent molecular mass of the rmGOL1 is approximately 25 and 48 kDa in SDS-PAGE under reducing conditions, corresponding to the monomer and dimer respectively.

活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

内毒素：Please contact us for more information.

基因名称：glyoxalase I

通用名：GLO1

蛋白序参考：NP_079650.3

总结：Lactoylglutathione lyase, also known as Methylglyoxalase, Aldoketomutase, Glyoxalase I, Ketone-aldehyde mutase, S-D-lactoylglutathione methylglyoxal lyase and GLO1, is a member of the glyoxalase I family. GLO1 / Glyoxalase I is a ubiquitous cellular defense enzyme involved in the detoxification of methylglyoxal, a cytotoxic byproduct of glycolysis. Accumulative evidence suggests an important role of GLO1 expression in protection against methylglyoxal-dependent protein adduction and cellular damage associated with diabetes, cancer, and chronological aging. GLO1 / Glyoxalase I has been implicated in anxiety-like behavior in mice and in multiple psychiatric diseases in humans. GLO1 / Glyoxalase I catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. GLO1 / Glyoxalase I exists in three separable isoforms which originate from two alleles in the genome. These correspond to two homodimers and one heterodimer composed of two subunits showing different electrophoretic properties. GLO1 upregulation may play a functional role in glycolytic adaptations of cancer cells.

形式：Lyophilized

纯度：> 85 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：0610009E22Rik Protein, Mouse; 1110008E19Rik Protein, Mouse; 2510049H23Rik Protein, Mouse; AW550643 Protein, Mouse; Glo-1 Protein, Mouse; Glo-1r Protein, Mouse; Glo-1s Protein, Mouse; Glo1-r Protein, Mouse; Glo1-s Protein, Mouse; GLY1 Protein, Mouse; Qglo Protein, Mouse