Neuropilin-1 Protein, Mouse, R

蛋白长度：A DNA sequence encoding the mouse NRP1 (NP_032763.2) (Met1-Pro856) was expressed with the Fc region of mouse IgG2a at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Mouse

氨基酸序：Met1-Pro856

标签类型：C-mFc

分子量：The recombinant mouse NRP1 consists of 1068 amino acids and predicts a molecular mass of 120 kDa.

活性：Measured by its binding ability in a functional ELISA. Immobilized Mouse VEGF164 (Cat:PM52980I0) at 2 μg/ml (100 μl/well) can bind Mouse Neuropilin-1 mFc(Cat:PM52956M4), the ECof Mouse Neuropilin-1 mFc is 4.0-20.0 ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：neuropilin 1

通用名：NRP1

蛋白序参考：NP_032763.2

总结：Neuropilin is a type I transmembrane protein and the molecular mass is 120 kDa. Two homologs, Neuropilin-1 and Neuropilin-2, are identified. The primary structure of Neuropilin-1 and Neuropilin-2 is well conserved and is divided into four domains, CUB (a1/a2) domain, FV/FVIII (b1/b2) domain, MAM (c) domain, and (d) domain that contains a transmembrane and a short cytoplasmic region. Neuropilin-1 (NRP1) acts as a receptor for two different extracellular ligands, class 3 semaphorins, and specific isoforms of vascular endothelial growth factor. The functions of NRP1 and NRP2 have been extensively studied in neurons where they act in axon guidance and in endothelial cells where they promote angiogenesis and cell migration. Neuropilin-1 is likely to mediate contacts between the dendritic cells and the T lymphocytes via homotypic interactions and is essential for the initiation of the primary immune response. NRP1 is a co-receptor for VEGF receptor-2 (VEGFR2) that enhances the binding of VEGF165 to VEGFR2 and VEGF165-mediated chemotaxis. NRP1 expression is regulated in EC by tumor necrosis factor-alpha, the transcription factors dHAND and Ets-1, and vascular injury. NRP1 upregulation is positively correlated with the progression of various tumors. Overexpression of NRPI in rat tumor cells results in enlarged tumors and substantially enhanced tumor angiogenesis. On the other hand, soluble NRP1 (sNRP1) is an antagonist of tumor angiogenesis.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：C530029I03 Protein, Mouse; NP-1 Protein, Mouse; NPN-1 Protein, Mouse; Npn1 Protein, Mouse; Nrp Protein, Mouse