CD45 Protein, Mouse, Recombina

蛋白长度：A DNA sequence encoding the mouse PTPRC (AAA39458.1) (Arg453-Ser1152) was fused with five amino acids (DDDDK) at the C-terminus was expressed and purified.

性质：

表达系统：Baculovirus-Insect Cells

种属：Mouse

氨基酸序：Arg453-Ser1152

标签类型：No tag

分子量：The recombinant mouse PTPRC consists of 706 amino acids and has a calculated molecular mass of 81 kDa. The recombinant protein migrates as an approximately 93 kDa band in SDS-PAGE under reducing conditions.

活性：1. The specific activity was determined to be 9168 nmol/min/mg using p-nitrophenyl phosphate as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse PTPRC (453-1152) at 10 μg/ml (100 μl/well) can bind biotinylated human Galectin-1 (Cat:PH51598E0-E) with a linear range of 0.31-2.5 μg/ml.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：protein tyrosine phosphatase, receptor type, C

通用名：PTPRC

蛋白序参考：AAA39458.1

总结：The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.

形式：Liquid

纯度：> 85 % as determined by SDS-PAGE.

储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, 3mM DTT, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

别名：B220 Protein, Mouse; Cd45 Protein, Mouse; CD45R Protein, Mouse; L-CA Protein, Mouse; loc Protein, Mouse; Ly-5 Protein, Mouse; Lyt-4 Protein, Mouse; T200 Protein, Mouse