PCSK9 Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the human PCSK9 (NP_777596.2, with mutation Asp 374 Tyr) (Met1-Gln692) was expressed with a polyhistidine tag at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Gln692

标签类型：C-His

分子量：The recombinant human PCSK9 consists of 673 amino acids and predicts a molecular mass of 72.5 kDa.

活性：Measured by its binding ability in a functional ELISA. Immobilized Human LDLR mFc (Cat:PH52176M4) at 2 μg/ml (100 μl/well) can bind Human PCSK9(D374Y) His(Cat:PH52575M2), the ECof PCSK9（D374Y) His is 1-20 ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：proprotein convertase subtilisin/kexin type 9

通用名：PCSK9

蛋白序参考：NP_777596.2

总结：Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：FH3 Protein, Human; HCHOLA3 Protein, Human; LDLCQ1 Protein, Human; NARC-1 Protein, Human; NARC1 Protein, Human; PC9 Protein, Human