Jagged 1 Protein, Human, Recom

蛋白长度：A DNA sequence encoding the human JAG1 (NP_000205.1) extracellular domain (Met 1-Ser 1046) was expressed, fused with a polyhistidine tag at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Ser1046

标签类型：C-His

分子量：The recombinant human JAG1 consists of 1024 amino acids and has a predicted molecular mass of 112 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh JAG1 is approximately 160-180 kDa due to glycosylation.

活性：Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells.The EDfor this effect is typically 4-20 μg/mL.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：jagged 1

通用名：JAG1

蛋白序参考：NP_000205.1

总结：Protein Jagged 1, also known as JAG1, JAGL1, and CD339, is a single-pass type I membrane protein that contains 1 DSL domain and 15 EGF-like domains. JAG1/Jagged 1 is widely expressed in adult and fetal tissues. The expression of JAG1/Jagged 1 is up-regulated in cervical squamous cell carcinoma. JAG1/Jagged 1 is also expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. JAG1/Jagged 1 is a ligand for multiple Notch receptors. It is involved in the mediation of Notch signaling. JAG1/Jagged 1 may be involved in cell-fate decisions during hematopoiesis. JAG1/Jagged 1 seems to be involved in the early and late stages of mammalian cardiovascular development. It inhibits myoblast differentiation and enhances fibroblast growth factor-induced angiogenesis. Defects in JAG1/Jagged 1 are the cause of Alagille syndrome type 1 (ALGS1). Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. Defects in JAG1/Jagged 1 are also a cause of tetralogy of Fallot (TOF). TOF is a congenital heart anomaly that consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left), and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation.

形式：Lyophilized

纯度：> 85 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：AGS Protein, Human; AHD Protein, Human; AWS Protein, Human; CD339 Protein, Human; HJ1 Protein, Human; Jagged 1 Protein, Human; JAGL1 Protein, Human