PLA2G1B Protein, Human, Recomb

蛋白长度：A DNA sequence encoding the human PLA2G1B (NP_000919.1) (Met 1-Ser 148) with a C-terminal polyhistidine tag was expressed.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Ser148

标签类型：C-His

分子量：The recombinant human PLA2G1B comprises 144 amino acids after removal of the signal peptide, and has a predicted molecular mass of 16.2 kDa. rh PLA2G1B migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.

活性：Measured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrenedecanoyl)-sn-glycero-3-phosphomethanol.The specific activity is >2,500 pmol/min/μg.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：phospholipase A2, group IB (pancreas)

通用名：PLA2G1B

蛋白序参考：NP_000919.1

总结：phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein that belongs to the phospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.

形式：Lyophilized

纯度：> 92 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 10mM Tris, 5mM CaCl2, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：PLA2 Protein, Human; PLA2A Protein, Human; PPLA2 Protein, Human