PLK1 Protein, Human, Recombina

蛋白长度：A DNA sequence encoding the human PLK1 (NP_005021.2) (Met 1-Ser 603) was expressed, with a polyhistidine tag at the N-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Met1-Ser603

标签类型：N-His

分子量：The recombinant human PLK1 consists of 621 amino acids and predicts a molecular mass of 70.5 kDa. It migrates as an approximately 66 kDa band in SDS-PAGE under reducing conditions.

活性：The specific activity was determined to be ＞3 nmol/min/mg using casein as substrate.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：polo-like kinase 1

通用名：PLK1

蛋白序参考：NP_005021.2

总结：Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well-characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.

形式：Liquid

纯度：> 90 % as determined by SDS-PAGE.

储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Supplied as sterile 50mM Tris, 100mM NaCl, pH 7.4, 0.5mM EDTA, 0.5mM EGTA, 0.5mM PMSF, 25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

别名：PLK Protein, Human; STPK13 Protein, Human