MAP4K5 Protein, Human, Recombi

蛋白长度：A DNA sequence encoding the human MAP4K5 (NP_006566.2) (Met 1-Tyr 846) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Met1-Tyr846

标签类型：N-GST & His

分子量：The recombinant human MAP4K5/GST chimera consists of 1083 amino acids and has a calculated molecular mass of 123 kDa. It migrates as an approximately 116 kDa band in SDS-PAGE under reducing conditions.

活性：Kinase activity untested

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：mitogen-activated protein kinase kinase kinase kinase 5

通用名：MAP4K5

蛋白序参考：NP_006566.2

总结：Mitogen-activated protein kinase kinase kinase kinase 5, also known as Kinase homologous to SPS1/STE2, MAPK/ERK kinase kinase kinase 5, MEK kinase kinase 5, and MAP4K5, is a cytoplasm protein that belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE2 subfamily. MAP4K5 is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis, and prostate. It contains one CNH domain and one protein kinase domain. MAP4K5 is highly similar to yeast SPS1/STE2 kinase. Yeast SPS1/STE2 functions near the beginning of the MAP kinase signal cascades that are essential for yeast pheromone response. MAP4K5 has been shown to interact with CRKL and TRAF2. This kinase was shown to activate Jun kinase in mammalian cells. MAP4K5 is an early component of MAP kinase signal cascades. It may play a role in the response to environmental stress. MAP4K5 appears to act upstream of the JUN N-terminal pathway.

形式：Liquid

纯度：> 75 % as determined by SDS-PAGE.

储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM TCEP, pH 8.5 Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

别名：GCKR Protein, Human; KHS Protein, Human; KHS1 Protein, Human; MAPKKKK5 Protein, Human