- $4515
- Leading Biology
- 美国
- PH52338M1
- 2025年07月12日
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- 详细信息
- 技术资料
- 保存条件：
Stable for 1 year at -2358°C or below from date of shipment.
- 保质期：
1-2年
- 供应商：
安诺伦（北京）生物科技有限公司
- 规格：
100 ug
蛋白长度：A DNA sequence encoding the human RPRD1B (AAH33629.1) (Met1-Asp326) was expressed with an N-terminal polyhistidine tag.
性质：
表达系统：HEK293 Cells
种属：Human
氨基酸序：Met1-Asp326
标签类型：N-His
分子量：The recombinant human RPRD1B comprises 346 amino acids and has a predicted molecular mass of 39.3 kDa. The apparent molecular mass of the protein is approximately 39 kDa in SDS-PAGE under reducing conditions due to glycosylation.
活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称：regulation of nuclear pre-mRNA domain containing 1B
通用名：RPRD1B
蛋白序参考：AAH33629.1
总结：RPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator that enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A.
形式：Lyophilized
纯度：> 85 % as determined by SDS-PAGE.
储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
别名：C20orf77 Protein, Human; CREPT Protein, Human; dJ1057B20.2 Protein, Human; NET60 Protein, Human
性质：
表达系统：HEK293 Cells
种属：Human
氨基酸序：Met1-Asp326
标签类型：N-His
分子量：The recombinant human RPRD1B comprises 346 amino acids and has a predicted molecular mass of 39.3 kDa. The apparent molecular mass of the protein is approximately 39 kDa in SDS-PAGE under reducing conditions due to glycosylation.
活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称：regulation of nuclear pre-mRNA domain containing 1B
通用名：RPRD1B
蛋白序参考：AAH33629.1
总结：RPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator that enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A.
形式：Lyophilized
纯度：> 85 % as determined by SDS-PAGE.
储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
别名：C20orf77 Protein, Human; CREPT Protein, Human; dJ1057B20.2 Protein, Human; NET60 Protein, Human
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RPRD1B Protein, Human, Recombinant (His Tag)
$4515
