DCUN1D1 Protein, Human, Recomb

蛋白长度：A DNA sequence encoding the human DCUN1D1 (NP_065691.2) (Met1-Val259) was expressed with a polyhistidine tag at the N-terminus.

性质：

表达系统：E. coli

种属：Human

氨基酸序：Met1-Val259

标签类型：N-His

分子量：The recombinant human DCUN1D1 consists of 274 amino acids and predicts a molecular mass of 32 KDa. It migrates as an approximately 28-32 KDa band in SDS-PAGE under reducing conditions.

活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

内毒素：Please contact us for more information.

基因名称：DCN1, defective in cullin neddylation 1, domain containing 1

通用名：DCUN1D1

蛋白序参考：NP_065691.2

总结：DCUN1D1, also known as SCCRO, is part of an E3 ubiquitin ligase complex for neddylation. DCUN1D1 functions to recruit charged E2 and is involved in the release of inhibitory effects of CAND1 on cullin-RING ligase E3 complex assembly and activity. DCUN1D1 binds to the components of the neddylation pathway (Cullin-ROC1, Ubc12, and CAND1) and augments but is not required for cullin neddylation in reactions using purified recombinant proteins. DCUN1D1 also recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation in purified protein assays. DCUN1D1 also acts as am ncogene facilitating malignant transformation and carcinogenic progression.

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, 10% glycerol, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：DCNL1 Protein, Human; DCUN1L1 Protein, Human; RP42 Protein, Human; SCCRO Protein, Human; SCRO Protein, Human; Tes3 Protein, Human