IL-35 Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human IL27B (NP_005746.2) (Met1-Lys229) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimerwas purified.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Ser219 & Met1-Lys229

标签类型：

分子量：The recombinant human IL12A&IL27B comprises 905 (446+459) amino acids and has a calculated molecular mass of 102.1 (50.6+ 51.5) KDa. The apparent molecular mass of the recombinant human IL12A&IL27B is approximately 66 KDa respectively in SDS-PAGE under reducing conditions.

活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：interleukin 12A

通用名：IL12A & IL27B

蛋白序参考：NP_005746.2

总结：IL12A is a subunit of a cytokine that acts on T and natural killer cells and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A, together with IL27B, form a disulfide-linked heterodimer: IL12A&IL27B. IL12A&IL27B is required for the T-cell-independent induction of IFN-gamma and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of IL12A&IL27B in innate immunity.

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：CLMF Protein, Human; IL-12A Protein, Human; IL-35 Protein, Human; Interleukin-35 Protein, Human; NFSK Protein, Human; NKSF1 Protein, Human; P35 Protein, Human