CTLA-4 Protein, Human, Recombi

蛋白长度：A DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the a polyhistidine tag at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Phe162

标签类型：C-His

分子量：The recombinant human CTLA4 consists of 138 amino acids and has a predicted molecular mass of 15 kDa. As a result of glycosylation, the apparent molecular mass of human CTLA4 is approximately 22-27 kDa in SDS-PAGE under reducing conditions

活性：1. Immobilized Human CD86 hFc (Cat:PH50860M4) at 2 μg/mL (100 μL/well) can bind Human CTLA-4 His (Cat:PH52218M2), the ECof Human CTLA-4 His (Cat:PH52218M2) is 1.5-12.0 ng/mL. 2. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The EDfor this effect is 0.05-0.3μg/mL when stimulated with 1 μg/mL Recombinant Human B7 1/CD80.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：cytotoxic T-lymphocyte-associated protein 4

通用名：CTLA4

蛋白序参考：NP_005205.2

总结：Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：ALPS5 Protein, Human; CD Protein, Human; CD152 Protein, Human; CELIAC3 Protein, Human; CTLA-4 Protein, Human; GRD4 Protein, Human; GSE Protein, Human; IDDM12 Protein, Human