FGFR2 Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the human FGFR2 (NP_000132.3) cytoplasmic domain (Met 400-Thr 821) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Met400-Thr821

标签类型：N-GST & His

分子量：The recombinant human FGFR2 (aa 400-821) /GST chimera consists of 659 amino acids and predictes a molecular mass of 75.7 kDa. It migrates as an approximately 68 KDa band in SDS-PAGE under reducing conditions.

活性：1. The specific activity was determined to be 28 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate. 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR2 (aa 400-821) (Cat:PH52197I6) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF acidic (Cat:PH50214E0) with a linear range of 15.6-250 ng/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized recombinant recombinant human FGFR2 (aa 400-821) (Cat:PH52197I6) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF basic (Cat:10014-HNAE) with a linear range of 0.16-1.25 μg/ml.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：fibroblast growth factor receptor 2

通用名：FGFR2

蛋白序参考：NP_000132.3

总结：FGFR2, also known as CD332, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR2 acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. It is required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. FGFR2 plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. It also promotes cell proliferation in keratinocytes and imature osteoblasts, but promotes apoptosis in differentiated osteoblasts. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal CD332 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. Defects in CD3322 are the cause of Crouzon syndrome, Jackson-Weiss syndrome, Apert syndrome, Pfeiffer syndrome, Beare-Stevenson cutis gyrata syndrome, familial scaphocephaly syndrome, lacrimo-auriculo-dento-digital syndrome and Antley-Bixler syndrome without genital anomalies or disordered steroidogenesis.

形式：Liquid

纯度：> 90 % as determined by SDS-PAGE.

储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

别名：BBDS Protein, Human; BEK Protein, Human; BFR-1 Protein, Human; CD332 Protein, Human; CEK3 Protein, Human; CFD1 Protein, Human; ECT1 Protein, Human; JWS Protein, Human; K-SAM Protein, Human; KGFR Protein, Human; TK14 Protein, Human; TK25 Protein, Human