USP46 Protein, Human, Mouse, R

蛋白长度：A DNA sequence encoding the human / mouse USP46 [(Identical to the mouse USP46 (NP_808229.1)] (Met1-Glu366) was expressed. Human and Mouse USP46 sequences are identical.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human, Mouse

氨基酸序：Met1-Glu366

标签类型：No tag

分子量：The secreted recombinant human / mouse USP46 consists of 366 amino acids and predicts a molecular mass of 42.4 KDa. The apparent molecular mass of the protein is approximately 43 Kda in SDS-PAGE under reducing conditions due to glycosylation.

活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：ubiquitin specific peptidase 46

通用名：USP46

蛋白序参考：NP_808229.1

总结：USP46 belongs to the peptidase C19 family, USP12/USP46 subfamily. Deubiquitinating enzymes (DUBs) are a large group of proteases which are also commonly referred to as deubiquitinating peptidases, deubiquitinating isopeptidases, deubiquitinases, ubiquitin proteases, ubiquitin hydrolyases, ubiquitin isopeptidases, or Dubs. They regulate ubiquitin-dependent metabolic pathways by cleaving ubiquitin-protein bonds. They also may act as negative and positive regulators of the ubiquitin system. Besides ubiquitin recycling, they are also involved in processing of ubiquitin precursors, in proofreading of protein ubiquitination and in disassembly of inhibitory ubiquitin chains. USP46 is a deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. It may act by mediating the deubiquitination of GAD1/GAD67. USP46 has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have high activity and it is not involved in deubiquitination of monoubiquitinated FANCD2.

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：