NBL1 Protein, Human, Recombina

蛋白长度：A DNA sequence encoding the human DAN precursor (NP_005371.1) (Met 1-Asp 180) was expressed with C-terminal fused Fc region of human IgG1.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Asp180

标签类型：C-hFc

分子量：The recombinant human DAN/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and has a calculated molecular mass of 44.4 kDa. As a result of glycosylation, rh DAN/Fc monomer migrates as an approximately 55-60 kDa protein in SDS-PAGE under reducing conditions.

活性：Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The EDfor this effect is typically 0.2-1.2 μg/ml in the presence of 50 ng/mL of recombinant human BMP4.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：neuroblastoma 1, DAN family BMP antagonist

通用名：NBL1

蛋白序参考：NP_005371.1

总结：The Dan (Differential screening-selected gene aberrative in neuroblastoma, also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in the vertebrates. It is a founding member of the DAN family of secreted proteins, acts as an inhibitor of cell cycle progression, and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante, and PRDC (protein related to DAN and Cerberus), and share the C-terminal cystine-knot motif. As a secreted glycoprotein, DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes, and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in the regulation of the entry of cells into the S phase. Besides, the DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumor suppressor gene for neuroblastoma.

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：D1S1733E Protein, Human; DAN Protein, Human; DAND1 Protein, Human; NB Protein, Human; NO3 Protein, Human