Brk Protein, Human, Recombinan

蛋白长度：A DNA sequence encoding the full length of human PTK6 (NP_005966.1) (Met 1-Thr 451) was fused with the GST tag at the N-terminus.

性质：

表达系统：Baculovirus-Insect Cells

种属：Human

氨基酸序：Met1-Thr451

标签类型：N-GST

分子量：The recombinant human GST/PTK6 chimera consists of 676 amino acids and predicts a molecular mass of 78 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.

活性：Kinase activity untested

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：protein tyrosine kinase 6

通用名：PTK6

蛋白序参考：NP_005966.1

总结：Tyrosine kinase (PTKs) is a protein that carries out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demonstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase that may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.

形式：Liquid

纯度：> 89 % as determined by SDS-PAGE.

储存条件：Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

别名：BRK Protein, Human