KLK4 Protein, Human, Recombina

蛋白长度：A DNA sequence encoding the human KLK4 (NP_004908.3) (Met 1-Ser 254) was expressed, with a polyhistidine tag at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Ser254

标签类型：C-His

分子量：The secreted recombinant human KLK4 consists of 239 amino acids and predictes a molecular mass of 25.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhKLK4 is approximately 30-35 kDa due to glycosylation.

活性：Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. (R&D Systems, Catalog # ES011). The specific activity is >250 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：kallikrein-related peptidase 4

通用名：KLK4

蛋白序参考：NP_004908.3

总结：Kallikrein-4, also known as Enamel matrix serine proteinase 1, Kallikrein-like protein 1, KLK-L1, Serine protease 17, KLK4, PRSS17, and EMSP1, is a secreted protein that belongs to the peptidase S1 family and Kallikrein subfamily. Kallikrein-4 / KLK4 is a serine protease expressed during enamel maturation, and proteolytic processing of the enamel matrix by KLK4 is critical for proper enamel formation. Kallikrein-4 / KLK4 contains one peptidase S1 domain. Kallikrein-4 / KLK4 is secreted by transition- and maturation-stage ameloblasts. KLK4 aggressively degrades the retained organic matrix following the termination of enamel protein secretion. Two proteases are secreted into the enamel matrix of developing teeth. The early protease is enamelysin (MMP-2). The late protease is kallikrein 4 (KLK4). The principal functions of MMP-2 and KLK4 in dental enamel formation are to facilitate the orderly replacement of organic matrix with mineral, generating an enamel layer that is harder, less porous, and unstained by retained enamel proteins. Defects in Kallikrein-4 / KLK4 are the cause of Amelogenesis Imperfecta Hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.

形式：Lyophilized

纯度：> 94 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：AI2A1 Protein, Human; ARM1 Protein, Human; EMSP Protein, Human; EMSP1 Protein, Human; kallikrein Protein, Human; KLK-L1 Protein, Human; PRSS17 Protein, Human; PSTS Protein, Human