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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Stable for 1 year at -1629°C or below from date of shipment.
- 保质期:
1-2年
- 供应商:
安诺伦(北京)生物科技有限公司
- 规格:
100 ug
性质:
表达系统:HEK293 Cells
种属:Human
氨基酸序:Met1-Asp189
标签类型:C-His
分子量:The recombinant human IFNA10 consists of 177 amino acids and predicts a molecular mass of 20.9 kDa.
活性:Measured in antiviral assays using WISH cells infected with vesicular stomatitisvirus (VSV). The EDfor this effect is 0.3-3 pg/mL.
内毒素:< 1.0 EU per μg protein as determined by the LAL method.
基因名称:interferon, alpha 10
通用名:IFNA10
蛋白序参考:NP_002162.1
总结:Interferon alpha-10 (IFNA10) is a member of the interferon family. Interferons belong to the group of the regulatory glycoproteins, of low molecular mass. They are the products of infected cell-genome, but not virus, as a consequence of the cause answer by different inductors. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They allow communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. IFNs have other functions: they activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes, and they increase the ability of uninfected host cells to resist new infection by the virus. Certain host symptoms, such as aching muscles and fever, are related to the production of IFNs during infection. Human IFNs are divided on the sequence of amino-acids into three groups: Alpha, Beta, and Gamma interferons.
形式:Lyophilized
纯度:> 95 % as determined by SDS-PAGE.
储存条件:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
别名:IFN-alphaC Protein, Human; MGC119878 Protein, Human; MGC119879 Protein, Human
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文献和实验Protein Engineering of Interferon Alphas
differences in these proteins may be responsible for a significant variety of biological actions. Understanding the structure and function of human IFN-α is very important. Recombinant techniques are important tools for the production and modification of IFN
motifs located prevalently in the 3′ untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based
High Throughput Production of Recombinant Human Proteins for Crystallography
, to be applied based on specific knowledge of protein families or when the core protocol is unsatisfactory. This scheme has been applied to more than 550 human proteins (x003E;10,000 constructs) and has resulted in the deposition of 112 unique structures
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