ACVR1 Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the extracellular domain (Met 1-Val 124) of human ALK2 (NP_001104537.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Val124

标签类型：C-hFc & His

分子量：The recombinant human ALK2/Fc is a disulfide-linked homodimeric Protein after removal of the signal peptide. Each monomer consists of 352 amino acids and predicts a molecular mass of 39.6 kDa. As a result of glycosylation, the rh ALK2/Fc monomer migrates as approximately 40-45 kDa band in SDS-PAGE under reducing conditions.

活性：Measure by its ability to bind with human BMP2 in a functional ELISA.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：activin A receptor, type I

通用名：ACVR1

蛋白序参考：NP_001104537.1

总结：ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP).

形式：Lyophilized

纯度：> 95 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：ACTRI Protein, Human; ACVR1A Protein, Human; ACVRLK2 Protein, Human; ALK2 Protein, Human; FOP Protein, Human; SKR1 Protein, Human; TSRI Protein, Human