Stable for 1 year at -1566°C or below from date of shipment.
保质期:
1-2年
供应商:
安诺伦(北京)生物科技有限公司
规格:
50 ug
蛋白长度:A DNA sequence encoding the human PFKM (P08237-1) (Thr2-Val780) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
性质:
表达系统:Baculovirus-Insect Cells
种属:Human
氨基酸序:Thr2-Val780
标签类型:N-GST & His
分子量:The recombinant human PFKM /GST chimera consists of 1016 amino acids and has a calculated molecular mass of 112.9 kDa. The recombinant protein migrates as an approximately 113 kDa band in SDS-PAGE under reducing conditions.
活性:Kinase activity untested
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称:phosphofructokinase, muscle
通用名:PFKM
蛋白序参考:
总结:PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related to glycogen storage disease type VII, also identified as Tarui disease.
形式:Liquid
纯度:> 90 % as determined by SDS-PAGE.
储存条件:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerolPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.