Lipocalin-2/LCN2 Protein, Huma

蛋白长度：A DNA sequence encoding the human Lipocalin 2 precursor (NP_005555.2) (Met 1-Gly 198) was expressed with a C-terminal polyhistidine tag.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Gly198

标签类型：C-His

分子量：The secreted recombinant human LCN2 consists of 195 amino acids and predicts a molecular mass of 22.7 kDa. As a result of glycosylation, it migrates as an approximately 25.7 kDa protein in SDS-PAGE under reducing conditions.

活性：Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2. Recombinant human Lipocalin-2 can bind >1.5 μM of Fe(DHBA)3.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：lipocalin 2

通用名：LCN2

蛋白序参考：NP_005555.2

总结：Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a 25 kDa protein belonging to the lipocalin superfamily. It was initially found in activated neutrophils, however, many other cells, like kidney tubular cells, may produce NGAL in response to various insults. This protein is released from injured tubular cells after various damaging stimuli, is already known by nephrologists as one of the most promising biomarkers of incoming Acute Kidney Injury (AKI). Recent evidence also suggests its role as a biomarker in a variety of other renal and non-renal conditions. Moreover, recent studies seem to suggest a potential involvement of this factor also in the genesis and progression of chronic kidney diseases. NGAL is the first known mammalian protein that specifically binds organic molecules called siderophores, which are high-affinity iron chelators. NGAL, first known as an antibacterial factor of natural immunity, and an acute-phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation, NGAL has a clear pro-tumoral effect, as has already been observed in different tumors (e.g. breast, stomach, esophagus, brain) in humans. In thyroid carcinomas, NGAL is strongly induced by NF-kB, an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development. Thus, Lipocalin-2 (LCN2/NGAL) has been implicated in a variety of processes including cell differentiation, proliferation, survival, and morphogenesis.

形式：Lyophilized

纯度：> 97 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：24p3 Protein, Human; Lipocalin-2 Protein, Human; MSFI Protein, Human; NGAL Protein, Human; p25 Protein, Human