Stable for 1 year at -1236°C or below from date of shipment.
保质期:
1-2年
供应商:
安诺伦(北京)生物科技有限公司
规格:
20 ug
蛋白长度:A DNA sequence encoding the human cystatin E/M protein (NP_001314.1) (Met 1-Met 149) was expressed with a C-terminal polyhistidine-tag.
性质:
表达系统:HEK293 Cells
种属:Human
氨基酸序:Met1-Met149
标签类型:C-His
分子量:The mature form of recombinant human cystatin E/M consists of 132 amino acids and predicts a molecular mass of 15 kDa. It exists as two major forms with an identical N-terminal sequence. As a result of glycosylation, rh cystatin E/M migrates as doublets with the molecular mass of approximately 15 and 21 kDa respectively in SDS-PAGE under reducing conditions.
活性:At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA.When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate.We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method.
基因名称:cystatin E/M
通用名:CST6
蛋白序参考:NP_001314.1
总结:Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17 kDa) and an unglycosylated (14 kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.
形式:Lyophilized
纯度:> 95 % as determined by SDS-PAGE.
储存条件:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
储存溶液:Lyophilized from sterile 25mM MES, 0.15M NaCl, pH 6.5Please contact us for any concerns or special requirements.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.Please refer to the specific buffer information in the hard copy of CoA.