Thrombopoietin Protein, Human,

蛋白长度：A DNA sequence encoding the mature form of human THPO (NP_000451.1) (Ser 22-Gly 353) was fused with a polyhistidine tag at the N-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Ser22-Gly353

标签类型：N-His

分子量：The recombinant human THPO consists of 348 amino acids with the predicted molecular mass of 37.6 kDa. As a result of glycosylation, rhTHPO migrates as an approximately 78.2 kDa band in SDS-PAGE under reducing conditions.

活性：Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The EDfor this effect is typically 10-60 ng/ml.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：thrombopoietin

通用名：THPO

蛋白序参考：NP_000451.1

总结：Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action.

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：MGDF Protein, Human; MKCSF Protein, Human; ML Protein, Human; MPLLG Protein, Human; THCYT1 Protein, Human; TPO Protein, Human