p19 INK4d Protein, Human, Reco

蛋白长度：A DNA sequence encoding the human CDKN2D (P55273) (Met 10Leu 166) was fused with the GST tag at the N-terminus.

性质：

表达系统：E. coli

种属：Human

氨基酸序：Met10-Leu166

标签类型：N-GST

分子量：The recombinant human CDKN2D/GST chimera consists of 400 amino acids and has a predicted molecular mass of 44.9 kDa. It migrates as an approxiamtely 46 kDa band in SDS-PAGE under reducing conditions.

活性：Immobilized human GST-CDKN2D at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4 (Cat:PH52046I6), The ECof biotinylated human GST-CDK4 (Cat:PH52046I6) is 0.52-1.2 μg/ml.

内毒素：Please contact us for more information.

基因名称：cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4)

通用名：CDKN2D

蛋白序参考：

总结：Cyclin-dependent kinase inhibitor 2D(also known as CDKN2D or p19ink4d), a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, negatively regulates the cyclin D-CDK4/6 complexes, which promote G1/S transition by phosphorylating the retinoblastoma tumor-suppressor gene product. It is clearly shown that DNA repair is the main target of p19ink4d effect and that diminished apoptosis is a downstream event. Experiments has uncovered a role of p19INK4d as a regulator of DNA-damage-induced apoptosis and suggest that it protects cells from undergoing apoptosis by allowing a more efficient DNA repair. It has been demonstrated that p19INK4d expression enhances cell survival under genotoxic conditions. Previous work has shown that inactivation of the cyclin-dependent kinase inhibitor (CKI) p19(Ink4d) leads to progressive hearing loss attributable to inappropriate DNA replication and subsequent apoptosis of hair cells. It may also involved in male reproductive function including testicular atrophy, alteration in serum follicle stimulating hormone, qualitative increase in germ cell apoptosis, and delayed kinetics of meiotic prophase markers.

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：INK4D Protein, Human; p19 Protein, Human; p19-INK4D Protein, Human