B4GALT1 Protein, Human, Recomb

蛋白长度：A DNA sequence encoding the human B4GALT1 extracellular domain (NP_001488.2) (Gly 44-Ser 398) was fused with a polyhistidine tag at the N-terminus.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Gly44-Ser398

标签类型：N-His

分子量：The secreted recombinant human B4GALT1 consists of 371 amino acids and predicts a molecular mass of 41.5 kDa. By SDS-PAGE under reducing conditions, rh B4GALT1 migrates as an approximately 45-55 kDa protein due to glycosylation.

活性：At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

内毒素：< 1.0 EU per μg of the protein as determined by the LAL method.

基因名称：UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1

通用名：B4GALT1

蛋白序参考：NP_001488.2

总结：Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.

形式：Lyophilized

纯度：> 92 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：B4GAL-T1 Protein, Human; beta4Gal-T1 Protein, Human; CDG2D Protein, Human; GGTB2 Protein, Human; GT1 Protein, Human; GTB Protein, Human