TIGIT Protein, Human, Recombin

蛋白长度：A DNA sequence encoding the human TIGIT (NP_776160.2) (Met1-Pro141) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

性质：

表达系统：HEK293 Cells

种属：Human

氨基酸序：Met1-Pro141

标签类型：C-hFc & AVI

分子量：The recombinant human TIGIT consists of 373 amino acids and predicts a molecular mass of 41.6 kDa. As a result of glycosylation, it migrates as an approximately 49.2 kDa band in SDS-PAGE under reducing conditions.

活性：Measured by its binding ability in a functional ELISA. Immobilized human CD155/PVR His (Cat: PH50647M2) at 2 μg/mL (100 μl/well) can bind Human TIGIT (hFc & AVI Tag), Biotinylated (Cat: PH51006M4)，The ECof bind Human TIGIT (hFc & AVI Tag), Biotinylated is 10-60 ng/mL.

内毒素：< 1.0 EU per μg protein as determined by the LAL method.

基因名称：T cell immunoreceptor with Ig and ITIM domains

通用名：TIGIT

蛋白序参考：NP_776160.2

总结：TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.

形式：Lyophilized

纯度：> 90 % as determined by SDS-PAGE.

储存条件：In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

储存溶液：Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

别名：VSIG9 Protein, Human; VSTM3 Protein, Human; WUCAM Protein, Human